CBSE +2 Biotechnology

CLASS XII
(THEORY)
One paper Time: 3Hours Total Marks : 70
Unit V: Protein and Gene Manipulation Marks 40
Chapter I: Protein Structure and Engineering 15 Marks
Introduction to the world of Proteins
3-D Shape of Proteins
Structure Function relationship in Proteins
Purification of Proteins
Characterization of Proteins
Protein based products
Designing Proteins
Proteomics
Chapter II: Recombinant DNA Technology 15 Marks
Introduction
Tools of rDNA Technology
Making Recombinant DNA
DNA Library
Introduction of Recombinant DNA into host cells
Identification of recombinants
Polymerase Chains Reaction (PCR)
DNA Probes
Hybridization Techniques
DNA Sequencing
Site-directed mutagenesis
Chapter III: Genomics and Bioinformatics 10 Marks
Introduction
Genome Sequencing Projects
Gene Prodiction and counting
Genome similarity, SNP's and comparative genomics
Functional Genomics
History of Bioinformatics
Sequences and Nomenclature
Information Sources
Analysis using Bioinformatics tools.
Unit VI : Cell Culture Technology 30 Marks
Chapter I: Microbial Culture and Applications
Introduction
Microbial Culture Techniques
Measurement and Kinetics of microbial Growth
Scale up of microbial process
Isolation of microbial products
Strain isolation and Improvement
Applications of microbial culture technology
Bioethics in microbial technology
Chapter II: Plant Cell Culture and Applications
Introuction
Cell and Tissue Culture Techniques
Applications of Cell and Tissue Culture
Gene Transfer Methods in Plants
Transgenic Plants with Beneficial Traits
Diagnostics in Agriculture and Molecular Breeding
Bioethics in Plant Genetic Engineering
Chapter III: Animal Cell Culture and Applications 10 Marks
Introduction
Animal Cell Culture Techniques
Characterisation of Cell Lines
Scale-up of Animal Culture Process
Applicationsof Animal Cell Culture
Stem Cell Technology
Bioethics of Genetic Engineering in Animals
Practicals
Note: Every student will be required to do the following experiments during the academic
session102
List of Experiments
1. Isolation of bacterial plasmid DNA and its detection by gel eletrophoresis
2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid
4. Data retrival and data base search using internet site NCBI
5. Download a DNA and protein sequence from internet, analyse and comment on it.
6. Cell viability assay (using Evans blue Stain)
7. Determination of blood groups.
8. Estimation of DNA
9. Ion-exchange chromatography for proteins.
10. Reading of a DNA sequencing gel and arrive at the sequence.
11. Estimation of blood glucuse by enzymatic method (GOD/POD)
12. Project work.
Scheme of Evaluation:
Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
A. Two experiments : 6+6 (only one computer bassed practical)
Practical record : 04
Viva on Practicals : 04
B. Project work :
Write up : 05
Viva on project : 05
Total 30
Recommended Books:
1. A Textbook of Biotechnology-Class XI: published by CBSE, New Delhi.
2. A Laboratory Mannual of Biotechnology-Class XI:published by CBSE, New
Delhi.
3. A Textbook of Biotechnology-Class XII: published by CBSE, New Delhi
4. A Laboratory Mannual of Biotechnology-ClassXII: published by CBSE, New
Delhi.