CBSE +1 Biotechnology

Course Structure
Class XI
(Theory)
One Paper (Three Hours) 70 Marks
Unit I Introduction to Biotechnology 10
Fundamentals of Biochemical Engineering
Biotechnology and Society
Unit II Biomolecules 20
Building Blocks of Biomolecules-Structure and dynamics
Structure and function of Macromolecules.
Biochemical TechniquesUnit III Cell and Development 20
The basic unit of life
Cell Growth and development
Cellular Techniques
Unit IV Genetics and Molecular Biology 20
Principles of Genetics
Genome Function
Genetical Techniques
PRACTICALS
Note : Every student is required to do the following experiments during the academic
session.
1. Preparation of buffers and pH determination.
2. Sterlization techniques (Wet and Dry Sterlization, Chemical sterlization and
Utrafiltration.
3. Media preparation (Solid and Liquid LB medium)
4. Isolation of bacteria from curd and staining of bacteria.
5. Determination of bacterial growth curve.
6. Study of various stages of mitosis and calculation of mitotic index.
7. Preparation of Karyotype.
8. Cell counting (using Haemocytometer)
9. Isolation of genomic DNA.
10. Detection of DNA by gel electrophoresis.
11. Isolation of milk protein (casein)
12. Estimation of protein by Biuret method.
13. Assaying the enzyme acid phosphate.
Scheme of Evaluation:
Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of session will be as under:
Two experiments : 20 Marks
Viva on experiments : 5 Marks
Practical record : 5 Marks